Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Molecular Devices Filter Lens, 630 nm, Emission
The 630 nm filter lens is an interchangeable emission filter designed to enable red-region fluorescence detection on compatible microplate readers. It provides selective transmission centered at 630 nm to improve signal specificity for assays using red-emitting fluorophores.
- Center wavelength 630 nm.
- Designed for emission detection in the red spectral region.
- Interchangeable filter format for compatible microplate readers.
- Enhances signal specificity and reduces background for fluorescence assays.
- Single-unit form factor suitable for routine assay configuration changes.
Molecular Devices Fluorescence Polarization Filter, 625 nm, 35 nm, Horizontal
Molecular Devices fluorescence polarization emission filter with holder provides a 625 nm center wavelength and a 35 nm bandpass tailored for polarized fluorescence assays. The polarized horizontal orientation supports fluorescence polarization measurements and installs in compatible microplate readers to enable reliable polarized emission detection.
- Provides 625 nm center wavelength for emission detection.
- 35 nm bandpass delivers defined spectral selectivity.
- Polarized horizontal orientation for fluorescence polarization assays.
- Includes holder for installation in compatible filter carousels or slides.
- Suitable for use in polarized fluorescence and fluorescence polarization assays.
Molecular Devices Photometric Filter, 570 nm, Absorbance, Replacement
AD 340 photometric filter for 570 nm absorbance measurements. This replacement optical filter isolates the 570 nm band for photometric assays, improving wavelength specificity and measurement reliability in absorbance-based detection systems.
- Center wavelength 570 nm for absorbance measurements.
- Designed for photometric wavelength selection in plate readers.
- Helps improve assay specificity and reduce spectral interference.
- Compact optical filter form factor for easy installation and replacement.
- Suitable for routine maintenance and filter replacement in absorbance detection workflows.
Molecular Devices Photometric Absorbance Filter, 470 nm, 8 nm Bandwidth
The AD 340 photometric filter centered at 470 nm is an absorbance accessory designed for narrowband photometric measurements in microplate assays. It delivers wavelength-specific absorbance detection for filter-based workflows and is intended for compatible multimode microplate readers.
- Absorbance filter for photometric measurements.
- Center wavelength 470 nm.
- Bandwidth approximately 8 nm.
- Designed for use with F3 and F5 multimode microplate readers.
- Suitable for filter-based absorbance assays in microplates.
Molecular Devices Time Resolved Filter, 340 nm, 70 nm bandwidth
Time-resolved excitation filter with a 340 nm center wavelength and a 70 nm bandwidth supplied with a holder for installation in compatible filter modules. Designed for time-resolved fluorescence and TR-FRET measurements in multi-mode microplate readers.
- Supports time-resolved fluorescence and TR-FRET assays.
- 340 nm center wavelength with 70 nm bandwidth.
- Includes filter holder for module installation.
- Compatible with enhanced TRF filter modules and multi-mode microplate readers.
- Optical construction for reproducible excitation performance.
Molecular Devices Fluorescence Excitation Filter, 535 nm, 25 nm bandwidth
Molecular Devices 535 nm fluorescence excitation filter (MPN 6590-0057) is a bandpass excitation filter centered at 535 nm with a 25 nm bandwidth, intended for installation in compatible microplate reader filter slides to provide selective excitation for fluorescence assays.
- Excitation bandpass centered at 535 nm with a 25 nm bandwidth.
- Optimized for fluorescence assay excitation to improve signal specificity.
- Form factor designed for installation in compatible microplate reader filter slides.
- Intended for use with multi-mode microplate readers that accept filter slides.
Molecular Devices Low-Volume Slide Refill Kit, 64-Well, Pack of 3
Replenishment kit of low-volume bottom slides for slide-based microplate readers. The kit includes three low-volume bottom slides, each with 64 wells, intended for low-volume absorbance and related assays.
- Contains three 64-well low-volume bottom slides.
- Intended for low-volume absorbance and slide-based assays.
- Supplies replacement slides to restore slide reader capacity.
Molecular Devices Reader Cable, MAC-Series 1, Emax and Vmax Compatible
Molecular Devices replacement cable designed to connect MAC-Series 1 readers to compatible Emax and Vmax microplate readers for instrument control and data transfer.
- Replacement interconnect cable for legacy microplate readers.
- Enables data and control signal transfer between instruments.
- Plug-and-play design for straightforward installation.
- Supplied as a single unit for spare parts inventory.
Molecular Devices Photometric Filter, 540 nm, 8 nm Bandwidth
Molecular Devices AD 340 photometric filter provides a narrow-band absorbance measurement centered at 540 nm for compatible multimode microplate readers. It is intended as a replacement or accessory filter for routine absorbance assays and instrument maintenance.
- Provides a 540 nm center wavelength for absorbance measurements.
- 8 nm full width at half maximum (bandwidth) for narrow-band detection.
- Bandpass design reduces out-of-band signal and improves assay specificity.
- Designed for use with compatible multimode microplate readers; verify instrument compatibility before ordering.
- Packaged as a single replacement filter for instrument maintenance and routine swaps.
Molecular Devices Fluorescence Excitation Filter, 370 nm, Time-Resolved, Single Unit
Molecular Devices 370 nm time-resolved fluorescence filter provides excitation at 370 nm for time-resolved fluorescence assays. It is supplied as a single replacement optical filter for compatible fluorescence instruments.
- Provides excitation centered at 370 nm.
- Designed for time-resolved fluorescence measurements.
- Supplied as a single replacement optical filter.
- Intended for use in compatible fluorescence detection instruments.
- Helps improve excitation specificity for time-resolved assays.
Ace Glass, Inc. 100mL vessel with sloped shoulders and a #25 Ace-Thred neck
100mL vessel with sloped shoulders and a #25 Ace-Thred neck; includes PTFE lined cap. For use in freeze drying and storage. After drying material, vessel can be capped and stored. Working capacity of vessel is about 1/2 of volume.